Table 1 Properties of L1 and L2 variants.

From: Effect of non-repetitive linker on in vitro and in vivo properties of an anti-VEGF scFv

 

Flexibility score

Immunogenicity score

MW (kDa)

Monomer/dimer ratio (monomer %)

% Insoluble aggregates*

L1

0.47

−1.09

32.8

95.7 ± 0.7

88.5 ± 2.8

L2

0.53

−0.06

31.9

66.5 ± 3.2

90.2 ± 4.3

  1. *Percent insoluble aggregation was calculated by subtracting soluble protein concentration from total concentration after thermal (60 °C) and mechanical (220 rpm) stress for 4 h. The average of 3 different samples was used.
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