Figure 2

Differential Gene Regulation by AR isoforms: (a,b) Scatter plot illustrating genes regulated uniquely by AR or AR-V7, regulated by both or regulated in the opposite direction (eg: AR induced and AR-V7 repressed); colored by expression pattern. Data are plotted as log2 fold change of gene expression in R1881 vs vehicle treated (x-axis) and Dox vs vehicle (0.1% ethanol) treated (y-axis) LNCaP-AR-V7 cells (a) and VCaP AR-V7 cells (b). (c) Venn diagram showing number and overlap of AR and AR-V7 regulated genes in LNCaP and VCaP models. FC > 1.5, adjusted p < .05. Venn diagrams are plotted as circles proportional to the numbers. (d) Comparison of top Hallmark Pathways upregulated by AR (red) and AR-V7 (green) in LNCaP (left) and VCaP (right) cell models. (e) Gene expression profiles of genes specifically regulated by AR-V7, by both isoforms or by AR. (f) Venn diagram comparing the number of unique AR-V7 induced genes (left) or unique AR induced genes (right) (FC > 1.5, adjusted p < .05) in LNCaP-V7 cells that overlap with previously reported AR upregulated genes in C4-2B (FC > 1.25, adjusted p < .05)²⁶ and LNCaP ABL models (FC > 1.5, adjusted p < .05)²⁷ identified by depleting AR in cells in CSS (plots not proportional). (g) Venn diagram showing the number of unique AR-V7 (left) or AR (right) repressed genes (FC > 1.5, adjusted p < .05) in LNCaP-V7 cells overlapping AR regulated genes in C4-2B (FC > 1.25, adjusted p < .05) and LNCaP ABL models (FC > 1.5, adjusted p < .05) as above. (h) Venn diagram showing number of unique AR-V7 up-regulated genes in LNCaP AR-V7 cells overlapping previously reported AR-V7 signatures in LN95 and 22RV1 cells identified either by depleting AR-V7 (Cai et al.¹⁹) or all variants (He et al.¹⁷). Unique AR-V7 genes from LNCaP AR-V7 (FC > 1.5, adjusted p < .05) were compared with AR-V7 signature from 22RV1 and LN95 (FC > 1.25, adjusted p < .05). (i) Venn diagrams showing the down-regulated genes corresponding to the analysis in (h). All qPCR plots are mean of three biological replicates from the same experiment and error bars are SEM. Venn diagrams (f–i) are plotting symmetrical circles and not proportionate to numbers.