Table 1 Summary of the inflammatory response in bronchoalveolar lavage (BAL), blood/serum and PEx for segmental LPS challenge (V1–V4).
Visit | V1 | V2 | V3pre | V3post | V4 | |||
|---|---|---|---|---|---|---|---|---|
Segmental challenge | ||||||||
Baseline | Saline | LPS | ||||||
BAL | Macrophages | % | 87.8 (84.7; 90.2) | 85.8 (77.6; 91.0) | 12.4 (8.2; 15.6)*** | |||
Neutrophils | % | 1.1 (0.8; 2.4) | 3.0 (1.4; 5.6) | 64.0 (60.0; 67.3)*** | ||||
Lymphocytes | % | 7.7 (4.8; 9.0) | 6.3 (4.9; 7.1) | 2.3 (1.7; 3.4)*** | ||||
Monocytes | % | 1.7 (0.9; 1.9) | 2.0 (1.4; 3.1) | 22.6 (18.3; 26.3)*** | ||||
Eosinophils | % | 0.0 (0.0; 0.1) | 0.1 (0.0; 0.2) | 0.1 (0.0; 0.6) | ||||
Col. epithelial cells | % | 1.8 (1.4; 2.2) | 1.4 (0.9; 1.6) | 0.1 (0.0; 0.1)*** | ||||
Sq. epithelial cells | % | 0.1 (0.0; 0.3) | 0.0 (0.0; 0.1) | 0.0 (0.0; 0.0) | ||||
Macrophages | 104/mL | 5.8 (5.5; 9.6) | 7.7 (5.6; 10.5) | 8.6 (6.2; 11.1) | ||||
Neutrophils | 104/mL | 0.1 (0.1; 0.2) | 0.2 (0.1; 0.6) | 43.8 (33.2; 67.9)*** | ||||
Lymphocytes | 104/mL | 0.5 (0.4; 0.6) | 0.5 (0.4; 0.9) | 1.3 (1.1; 2.6)* | ||||
Monocytes | 104/mL | 0.1 (0.1; 0.2) | 0.2 (0.1; 0.3) | 13.9 (8.7; 28.6)*** | ||||
TCC | 104/mL | 6.6 (6.2; 11.7) | 8.5 (7.0; 11.7) | 68.2 (56.0; 99.7)*** | ||||
IL6 | pg/mL | 0.6 (0.4; 0.9) | 1.6 (0.9; 2.2) | 61.1 (39.9; 71.1)*** | ||||
IL8 | pg/mL | 12.2 (10.2; 15.8) | 16.8 (7.6; 22.3) | 52.2 (43.9; 65.9)*** | ||||
ALB | µg/mL | 36.9 (31.2; 39.7) | 33.6 (29.4; 41.9) | 143.5 (115.8; 185.7)*** | ||||
MPO | ng/mL | 5.2 (2.9; 9.1) | 12.3 (4.8; 20.1) | 100.5 (87.8; 141.2)*** | ||||
SPD | ng/mL | 217.6 (181.2; 242.5) | 211.3 (182.9; 256.8) | 175.4 (156.6; 202.6) | ||||
Blood | Neutrophils | % | 56.5 (53.3; 60.1) | 78.7 (73.8; 82.5)** | 64.7 (61.1; 70.8)** | |||
Lymphocytes | % | 32.1 (29.3; 34.4) | 13.1 (12.0; 18.2)** | 27.8 (20.8; 29.2)* | ||||
Monocytes | % | 8.8 (6.9; 9.6) | 6.8 (5.4; 7.2)* | 6.4 (4.9; 7.6)*** | ||||
Eosinophils | % | 1.6 (1.0; 2.7) | 0.9 (0.4; 1.3)** | 1.0 (0.6; 1.7)* | ||||
Basophils | % | 0.7 (0.5; 1.2) | 0.4 (0.3; 0.6)** | 0.6 (0.4; 0.6)* | ||||
Leukocytes | G/L | 5.0 (4.2; 6.0) | 10.6 (9.8; 12.3)*** | 7.6 (6.3; 8.4)*** | ||||
Erythrocytes | T/L | 4.8 (4.5; 5.2) | 4.7 (4.6; 4.9) | 4.8 (4.7; 5.1) | ||||
Hemoglobin | g/dL | 14.5 (13.3; 14.8) | 14.2 (13.8; 14.5) | 14.3 (14.1; 14.9) | ||||
Hematokrit | L/L | 0.4 (0.4; 0.4) | 0.4 (0.4; 0.4) | 0.4 (0.4; 0.4) | ||||
MCH | pg/Ery | 29.3 (28.9; 30.5) | 30.1 (29.0; 30.6) | 29.4 (29.0; 30.3) | ||||
MCHC | g/dL | 33.9 (33.7; 34.5) | 34.2 (33.7; 35.3) | 34.6 (34.0; 34.9) | ||||
MCV | fL | 86.9 (84.3; 88.5) | 87.1 (85.1; 88.6) | 86.7 (83.2; 88.3) | ||||
Thrombocytes | G/L | 222.0 (206.3; 232.3) | 220.5 (206.3; 249.0) | 218.5 (194.5; 229.0) | ||||
Serum | IL6 | pg/mL | 0.6 (0.5; 0.8) | 11.5 (6.0; 20.8)*** | 2.0 (1.5; 5.2)*** | |||
IL8 | pg/mL | 7.4 (4.2; 7.8) | 7.6 (4.6; 10.1) | 4.0 (3.1; 5.2)* | ||||
ALB | mg/mL | 71.5 (60.6; 77.9) | 75.8 (65.0; 80.9) | 75.0 (70.9; 77.8) | ||||
MPO | ng/mL | 12.8 (8.2; 16.4) | 37.5 (24.3; 67.2)** | 38.0 (24.4; 60.5)*** | ||||
SPD | ng/mL | 5.2 (3.6; 6.7) | 5.8 (4.0; 6.0) | 5.7 (3.9; 7.6) | ||||
PEx | No. Pex sampled | n | 19.0 (15.5; 25.8) | 13.0 (9.3; 21.5) | 10.5 (8.3; 12.8)* | 15.0 (12.5; 22.8) | ||
Vol breath sampled | L | 86.7 (68.3; 94.7) | 56.9 (42.4; 89.9) | 39.7 (36.2; 56.9)** | 75.8 (57.8; 96.6) | |||
Mass sampled | ng | 242.7 (157.6; 243.9) | 240.3 (228.2; 246.1) | 245.1 (240.4; 255.7) | 241.5 (238.6; 242.3) | |||
IL6 | fg/mL | 0.0 (0.0; 0.0) | 0.0 (0.0; 0.0) | 24.3 (14.7; 34.2)*** | 0.3 (0.1; 1.2)* | |||
IL8 | pg/mL | 0.04 (0.01; 0.04) | 0.02 (0.01; 0.04) | 0.06 (0.05; 0.10)** | 0.04 (0.02; 0.07) | |||
ALB | ng/mL | 29.8 (27.9; 35.4) | 31.3 (25.7; 34.1) | 23.9 (18.1; 25.3) | 31.3 (19.3; 34.5) | |||
MPO | pg/mL | 0.1 (0.1; 0.1) | 0.1 (0.1; 0.1) | 0.1 (0.1; 0.2) | 0.3 (0.1; 1.9) | |||
SPD | pg/mL | 38.4 (28.7; 48.6) | 34.1 (18.2; 37.9) | 22.9 (17.3; 38.9) | 22.5 (14.6; 31.0) | |||
