Figure 1
From: The impact of CCR8+ regulatory T cells on cytotoxic T cell function in human lung cancer
CCR8 expression on immune cells of lung cancer patients. Single cell suspensions isolated from lung tumor tissues, tumor-adjacent normal tissues and PBMCs were analyzed by flow cytometry. (A) FOXP3 and CCR8 expressions on CD4 T cells (gated on live CD45+ CD3+ CD4+ cells). (B) CCR8 expression on FOXP3+ or FOXP3− CD4 T cells. Representative flow cytometric data are shown. (C) Percentages of CCR8+ cells in FOXP3+ CD4 T, FOXP3− CD4 T, CD8 T, CD19+, CD16+, CD14+, CD15+, and CD11c+ cell subsets in lung tumors, tumor-adjacent normal tissues and PBMCs (n = 11). Data are shown as mean values ± SD. Statistical significance was determined by paired t-test (*p ≤ 0.05; **p < 0.01) compared with tumor infiltrating FOXP3+ CD4 T cell group.
