Figure 5
From: The impact of CCR8+ regulatory T cells on cytotoxic T cell function in human lung cancer
Impact of CCR8+ cell depletion on CD8 T cells in lung tumors. CCR8+ cells were magnetically removed from TICs of lung cancer and cultured with IL-2 and CpG. After 5 days, CD8 T cells in TICs were detected by flow cytometry. (A) Representative flow cytometric data (left) and the percentage of GZMB-expressing cells (right) in CD8T cells (gated on live CD45+ CD3+ CD8+ cells) are shown (n = 15). (B) Representative flow cytometric data (left) and percentage of IFNγ-producing cells (right) in CD8 T cells after PMA/ionomycin stimulation are shown (n = 15). (C) Percentages of IFNγ-producing cells in cultured CD8 T cells after CCR8+ cell and CCR4+ cell depletion (n = 5). (D) After depletion of CCR8+ cells, TICs were cultured in the presence of anti-HLA-A,B,C antibody for 5 days. Percentages of IFNγ-producing cells in CD8 T cells were detected by flow cytometry (n = 4). Lines connect data from the same donor samples. Statistical significance was determined by paired t-test (*p ≤ 0.05; **p < 0.01; ***p < 0.001).
