Table 2 Yolk lipid content (%) and fatty acid composition (expressed in % of total lipids) analysis just prior to (E0) and at day 10 (E10) of incubation in pHu+ and pHu− lines (N = 10).

	E0_ pHu+	E0_ pHu−	E10_ pHu+	E10_ pHu−	P-value
Total lipids	32.94 ± 0.92^a	27.71 ± 1.08^b	23.56 ± 2.25^bc	21.53 ± 1.80^c	0.0005
C14	0.33 ± 0.01	0.32 ± 0.006	0.35 ± 0.02	0.34 ± 0.01	0.36
C14:1	0.08 ± 0.003^ab	0.07 ± 0.006^ab	0.09 ± 0.006^a	0.07 ± 0.008^b	0.03
C16	24.97 ± 0.05	24.87 ± 0.18	25.49 ± 0.24	25.11 ± 0.28	0.27
C16:1	2.74 ± 0.07	2.58 ± 0.10	2.99 ± 0.15	2.74 ± 0.17	0.15
C18	7.33 ± 0.14	7.54 ± 0.17	7.39 ± 0.16	7.48 ± 0.21	0.83
C18:1	39.72 ± 0.41	39.54 ± 0.40	38.71 ± 0.49	39.11 ± 0.67	0.46
C18:2 n-6	20.77 ± 0.41	20.86 ± 0.35	21.06 ± 0.31	21.63 ± 0.38	0.40
C18:3 n-3	1.03 ± 0.05	1.02 ± 0.03	1.03 ± 0.04	1.04 ± 0.05	0.83
C20	0.02 ± 0,001	0.03 ± 0.006	0.03 ± 0.002	0.03 ± 0.003	0.43
C20:1	0.21 ± 0.009	0.21 ± 0.007	0.19 ± 0.007	0.21 ± 0.007	0.41
C20:4 n-6	1.62 ± 0.06	1.64 ± 0.07	1.55 ± 0.07	1.56 ± 0.09	0.77
C22:4 n-6	0.25 ± 0.02^b	0.33 ± 0.02^a	0.27 ± 0.02^ab	0.26 ± 0.009^b	0.01
C22:5 n-3	0.17 ± 0.01	0.19 ± 0.01	0.16 ± 0.01	0.15 ± 0.01	0.28
C22:6 n-3	0.78 ± 0.04	0.81 ± 0.05	0.70 ± 0.03	0.73 ± 0.05	0.21
SFA	32.65 ± 0.16	32.76 ± 0.30	33.25 ± 0.23	32.95 ± 0.27	0.31
MUFA	42.74 ± 0.42	42.40 ± 0.39	41.98 ± 0.39	42.12 ± 0.64	0.65
PUFA	24.62 ± 0.42	24.85 ± 0.40	24.77 ± 0.34	24.92 ± 0.68	0.97
n-6	22.64 ± 0.40	22,83 ± 0.36	22.88 ± 0.31	22.99 ± 0.60	0.95
n-3	1.98 ± 0.04	2.02 ± 0.06	1.89 ± 0.04	1.93 ± 0.08	0.42
n-6/n-3	11.47 ± 0.22	11.40 ± 0.27	12.13 ± 0.17	12.00 ± 0.25	0.06

Data are presented as mean ± s.e.m. Mean values without a common letter (a, b, c) differ between groups. Mean comparisons were analysed by ANOVA or the Kruskal–Wallis test. FA: fatty acids; SFA: saturated fatty acids; MUFA: monounsaturated fatty acids; PUFA: polyunsaturated fatty acid.
Significant values are in bold.

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