Figure 3
From: Stent-based delivery of AAV2 vectors encoding oxidation-resistant apoA1
Impact of AAV2-apoA1(WT) and AAV2-apoA1(4WF) transduction on the proliferation and migration of SMC and BOEC, reactive oxygen species (ROS) production, and anti-inflammatory responses in endothelial cells. WST-8 assay was used to evaluate the effect of apoA1(WT) and apoA1(4WF) overexpression on proliferation of (A) TNFα (20 ng/ml)-stimulated rat aortic SMC and (B) non-stimulated rat BOEC. The results (both A and B) were normalized by WST-8 assay values produced in confluent cultures of rat SMC (A) and rat BOEC (B) and expressed as the percent of a monolayer confluency. A monolayer scratch injury assay was used to assess migratory capacity of apoA1(WT)- and apoA1(4WF)-transduced rat SMC (C) and rat BOEC (D) compared with non-transduced counterparts. A closure of the gap inflicted by pipette tip was quantified 24 h after the scratch injury. (E) CM-H2DCFDA assay in non-transduced, apoA1(WT)- and apoA1(4WF)-transduced RAEC with/without TNFα stimulation. (F) Attachment of PKH26-labeled rat monocytes to TNFα-stimulated RAEC.
