Figure 1

Volcano plot of miRNA expression in aqueous humor from subjects with pseudoexfoliation glaucoma and NTG compared to unaffected controls. (a) A total of 334 mature miRNAs were identified in the AH of PEX patients. Up-regulated miRNAs are shown to the right region of the plot (red) and down-regulated miRNAs are shown to the left region of the plot (green). Of these, two miRNAs, hsa-miR-30d-5p and hsa-miR-320a, were significantly up-regulated (fold-change > 2, p < 0.05) and ten miRNAs (hsa-miR-3156-5p, hsa-miR-4458, hsa-miR-6717-5p, hsa-miR-6728-5p, hsa-miR-6834-5p, hsa-miR-6864-5p, hsa-miR-6879-5p, hsa-miR-877-3p, hsa-miR-548e-3p, and hsa-miR-6777-5p) were significantly down-regulated (fold-change < − 2, p < 0.05) compared to controls. (b) In NTG patients, a total of 291 mature miRNAs were identified in the AH. Among these, ten miRNAs (hsa-let-7a-5p, hsa-let-7c-5p, hsa-let-7f.-5p, hsa-miR-192-5p, hsa-miR-10a-5p, hsa-miR-10b-5p, hsa-miR-375, hsa-miR-4510, hsa-let-7b-3p, and hsa-miR-222-3p) were significantly up-regulated (fold-change > 2, p < 0.05) and two miRNAs (hsa-miR-4639-5p and hsa-miR-6777-5p) were significantly down-regulated (fold-change < − 2, p < 0.05) compared to controls. Plots were prepared with ExDEGA v1.2.1.0 software. NTG: normal tension glaucoma, AH: aqueous humor, PEX: pseudoexfoliation.