Figure 7
(A–D) Intravital fluorescent microscopic images of endometriotic lesions (broken lines = borders) on day 0 (A,B) and day 14 (C,D) after transplantation of freshly isolated (0 h; A,C) and 24-h-precultivated (B,D) endometrial fragments from C57BL/6-TgN(ACTB-EGFP)1Osb/J donor mice into the dorsal skinfold chamber of C57BL/6J recipient mice. Microscopy was performed in blue light epi-illumination without (A,B) or with 5% FITC-labeled dextran for contrast enhancement by staining of plasma (C,D). Scale bars: 270 µm. (E,F) Functional capillary density (E, cm/cm2) and size (F, mm2) of endometriotic lesions directly after transplantation (d0) as well as on days 3, 6, 10 and 14 day after transplantation of freshly isolated (0 h; white bars; n = 5) and 24-h-precultivated (grey bars; n = 7) endometrial fragments from C57BL/6-TgN(ACTB-EGFP)1Osb/J donor mice into dorsal skinfold chambers of C57BL/6J recipient mice. Mean ± SEM. *P < 0.05 vs. 0 h.
