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Figure 1

From: Different methods of detaching adherent cells and their effects on the cell surface expression of Fas receptor and Fas ligand

Figure 1

The effects of different cell detachment buffers on surface protein expression in RAW264.7 macrophages. (a,c) The surface expression of Fas ligand (FasL) and Fas receptor was detected by flow cytometry in RAW264.7 murine macrophages treated with either EDTA or accutase for 10 min. (b,d) Statistical analysis revealed a significant difference in surface FasL expression between the EDTA and accutase treatment groups. (e,f) Neither EDTA nor accutase buffer incubation affected surface F4/80 proteins in RAW264.7 cells (p = 0.2). (G,H) The EDTA cell detachment buffer had minor effects on cell surface FasL expression when applied for 30 min compared to those of the cell scraping method. Cells incubated with the accutase cell detachment buffer for either 10 or 30 min exhibited significantly decreased surface FasL expression. (***p < 0.001, one-way ANOVA). MFI mean fluorescence intensity.

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