Figure 6
From: High-throughput translational profiling with riboPLATE-seq
Attenuation of Single-Drug Effects Under Combination Treatments in TS-543 cells. (a)–(c) Volcano plots of changes in RA observed under drug combinations, color-coded with the upregulated (red, magenta) and downregulated (blue, cyan) significant targets (DESeq2 FDR < 0.05, R v4.0.5) of each combination’s constituent drugs. In each plot, the X axis marks each gene’s observed difference in RA under drug combination treatment relative to DMSO controls, and the Y axis scales inversely with p value (-log10(FDR)) to correlate positively with the effect’s significance. Significant targets of both constituent drugs are attenuated in their effects in all combinations, indicated by significance values below the guideline y =  − log10(0.05) and effect sizes reduced towards x = 0, relative to their distributions in the volcano plots in Fig. 5 (by definition y >  − log10 (0.05)). (d)–(f) Scatterplots comparing the maximum observed effect in either individual drug on the X axis with its observed effect under combination treatment on the Y axis, for the set of genes significantly impacted by each drug combination’s two constituent drug treatments. Guidelines at Y = 0 and X = Y aid visualization of the attenuation in gene-specific effects of the individual drugs in combination. Most genes fall between Y = 0 and X = Y, indicating a lesser change in RA in the same direction under combination treatment compared with its maximum effect observed in singular treatments.
