Figure 4
From: Understanding of cell death induced by the constituents of Taxus yunnanensis wood
MIR34a binds to E2F3 mRNA in the Luciferase assay. (A–C) The ORF region of human E2F3 mRNA and the predicted binding site for MIR34a (the sequence region 4440–4462) were inserted into a pMIR-REPORT Luciferase miRNA Expression Reporter Vector (wild). The mutant-type pMIR vector contained a mutated seed sequence (from CTGC to AGTG) for MIR34a. Luciferase activities were measured after co-transfection with control RNA or MIR34a (10 nM) and wild or mutant pMIR vector.
