Figure 4
(a) Schematic illustration of S-TRAIL/GQD preparation steps. (b) Analysis of S-TRAIL content in centrifugation products (both supernatant and pellet) using polyacrylamide gel electrophoresis (12.5%), protein marker is 62KDa. (c) UV–visible spectra of S-TRAIL, GQDs and prepared S-TRAIL/GQD after centrifugation. (d) Fluorescence spectra of S-TRAIL, GQDs and S-TRAIL/GQD (pellet) and the supernatant upon excitation at 360 nm. (e) Fluorescence spectra of GQDs (6 µg/ml) in the presence of different concentrations of S-TRAIL upon excitation at 360 nm. (f) ζ-potential of S-TRAIL and GQD before and after conjugation. (g) Intrinsic fluorescence of S-TRAIL (160 µg/ml) in the presence of different concentrations of GQD upon excitation at 280 nm.
