Figure 4

Lenti-Spike inhibiting effects of imatinib were Abelson-kinases independent. (a) Lenti-Spike inhibition by imatinib is Abelson-kinases independent in TMPRSS2-negative cells. HEK293-KO cells for each Abl1, Abl2 or both, were transfected with hACE2 2 days before infection. Lenti-Spike infection is reduced in the same level in all the tested cell lines when treated with 10 µM imatinib at the time of infection. Results represent means of two independent experiments with two biological replicates each. (b) Abelson-kinases are not responsible for lenti-Spike inhibition with imatinib in TMPRSS2-positive cells. The Abl-KO cell lines were transfected with TMPRSS2 and hACE2 2 days before lenti-Spike infection. Results represent means of two independent experiments with two biological replicates each and student-t-test was applied; *p ≤ 0.05; **p ≤ 0.01.