Figure 3
From: Near infra-red labelling and tracking of corneal endothelial cells in-vivo
The effect of DIR on rabbit corneal endothelium following intracameral injection in vivo. DIR (final concentration 100 µg/ml) was injected into the anterior chamber of 3 rabbits to determine if this had any side-effects. (a) Photomicrograph of the rabbit anterior segment taken 1 day post DIR intracameral injection. The cornea remains transparent, with easy visualization of the iris details. (b) Infra-imaging using, the ICG filters on the Spectralis HRA, shows variable fluorescence across the endothelium, with speckled hyperfluorescent seen at areas of increased cellular dye uptake. (c) OCT image showing an optical cross-section of the central rabbit cornea. No increase in corneal thickness or inflammation is seen (CCT. Images from the same rabbit cornea taken on day 28 post DIR injection. (d) A photomicrograph of the rabbit anterior segment showing the cornea remains clear. (e) Infra-imaging using the ICG filters shows residual fluorescence at day 28, although maximal fluorescence intensity is reduced. (f) An OCT image taken at day 28 showing corneal thickness remains normal and unchanged throughout the study period (CCT 377 µm).
