Figure 5
From: Near infra-red labelling and tracking of corneal endothelial cells in-vivo
Comparison between in vivo DIR imaging and trypan blue staining. (a) Intra-operative trypan blue staining shows bare DM where endothelial cells have been removed by scraping. (b) The same eye was imaged immediately post-operatively. The DIR imaging of the area of denuded endothelial cells shows good concordance with intraoperative trypan blue (yellow outline), where DIR fluorescence is blocked by the nictitating membrane. At 3 days post-wounding, significant closure of the wound can be seen. (c) Injection of intracameral trypan blue stains a small residual area bare DM (yellow outline). A feathered edge is observed, representing migrating endothelial cells. (d) Infrared imaging of the same eye shows the same area of bare DM, surrounded by fluorescent endothelial cells (yellow outline). (e) An OCT image of the cornea at day 3 post wounding confirms DIR images are successfully acquired through thickened and irregular corneas. Scale bar: 1 mm.
