Figure 7

In vivo tracking of endothelial cells at pan-corneal and single-cell level. Images taken from an individual, DIR stained rabbit cornea. Following standard endothelial wounding, infra-red (a–d), OCT (e–h) and DIR images (i–p) were acquired using the HRA/Spectralis. Use of the anterior segment lens supplied by the manufacturer allowed macroscopic imaging of the whole cornea. By coupling additional lenses to the infinity optics retinal lens, higher magnification imaged could be captured (m–p). The images in the bottom row correspond to the areas seen in the white square outline in the macroscopic images. By sequentially imaging with increasing magnification, it was possible to track areas of interest. Immediately after scraping no DIR positive cells are seen in the wound area (i,m). At day 3 the cornea is still significant thicker that pre-wounding (f). The wound area can be seen to be much smaller (j, white box). At higher magnification levels, migrating cells closing the wound at the edge are seen (n). By day 5, although some residual corneal oedema and thickening persists on OCT imaging (g), complete wound closure can be observed (k,o). By day 14, corneal thickness has returned to normal (h) and a stable layer of DIR positive cells was observed with macroscopic (i) and higher magnification imaging (p).