Figure 5
From: Hydrocoel morphogenesis forming the pentaradial body plan in a sea cucumber, Apostichopus japonicus
The observed changes in cell shape and cell arrangement during the lobe formation in the hydrocoel. The cell membrane of larvae was stained with BODIPY FL C5-ceramide and observed using confocal laser scanning microscopy from phase 1 to phase 4. (a–f) Optical sections of the hydrocoel in each phase (ventral view, anterior to the top). The time course and stages are indicated in the figure. (a’–f’) show the magnification of the regions indicated in (a–f), respectively. Hydrocoel epithelium was a single cell layer in phase 1 and seemed to be multilayered in phase 2. In phase 3, the tissue became single layered again at the tip of the lobe (arrowhead in c’–d’) and then in the whole hydrocoel lobe.
