Figure 4

AKR1C3 expression by Protein Wes Simple in blood and bone marrow. (A) Increased expression of AKR1C3 by Protein Wes in peripheral blood specimens (indicated by red *) compared to the marrow aspirate specimens and control GAPDH expression. (B) In T-ALL cases with AKR1C3 expression in peripheral blood (PB) versus bone marrow aspirate (BMA), in which elevated expression is observed in PB samples regardless of their timepoint (Protein Wes Area t-test: P = 0.0277; Protein Wes height t-test: P = 0.0166). (C) When PB samples were segregated into diagnostic (PBdx) and samples of relapsed/refractory and/or minimal residual disease (PBR&R/MRD), PBR&R/MRD samples demonstrated elevated expression compared to BMA (Protein Wes Area ordinary one-way ANOVA: P = 0.0003).