Table 2 Proteins identified by LC–MS/MS in protein spots differentially expressed in Nellore bovine cecum fed on diets containing increasing levels of starch (25, 35 and 45%) and additives (monensin, blend essential oil + exogenous α-amylase).
Protein | Access | Score | pI/MM theoretical (Da) | pI/MM experimental (Da) |
|---|---|---|---|---|
Glucose and energy metabolism | ||||
Alpha-enolase | Q9XSJ4 | 1783.3310 | 6.37/47,326.13 | 6.70/56,906 |
Beta-enolase | Q3ZC09 | 440.2993 | 7.60/47,096.01 | 6.43/48,539 |
Triosephosphate isomerase | Q5E956 | 193.3130 | 6.45/26,689.51 | 7.24/25,458 |
l-lactate dehydrogenase B | Q5E9B1 | 4599.0320 | 6.02/36,723.64 | 6.37/39,211 |
l-lactate dehydrogenase A chain | P19858 | 1327.3960 | 8.12/36,597.64 | 6.37/39,211 |
Pyruvate kinase | A5D984 | 98.4805 | 7.96/57,948.91 | 5.9/57,613 |
Fructose-bisphosphate aldolase | A6QLL8 | 1850.8330 | 8.45/39,436.12 | 6.37/39,211 |
Phosphoglycerate mutase | F1N2F2 | 427.2343 | 9.01/28,699.04 | 6.37/39,211 |
l-lactate dehydrogenase | F1MK19 | 70.7983 | 5.72/36,724.58 | 6.37/39,211 |
Glyceraldehyde-3-phosphate dehydrogenase | P10096 | 11,907.1000 | 8.51/35,868.09 | 8.12/29,321 |
ATP synthase subunit beta_ mitochondrial | P00829 | 533.0471 | 5.15/56,283.53 | 5.49/47,920 |
Inflammatory response | ||||
Leukocyte elastase inhibitor | Q1JPB0 | 300.0084 | 5.70/42,235.75 | 5.70/38,338 |
