Figure 3

NOD-IPC transplantation. (a) Syngeneic model of NOD-IPCs transplantation. (b) Blood glucose (BG) concentration profile of NOD mice at the onset of diabetes after NOD-IPC transplantation. In the sham group (n = 4), the BG concentration remained high. However, the BG concentration gradually decreased after NOD-IPC transplantation to < 200 mg/dL at 10 days post-transplantation and stayed low until 30 days post-transplantation (n = 6). In three out of four recipient NOD mice, the BG concentration, which was improved by NOD-IPC transplantation, gradually increased to > 250 mg/dL 40–60 days after transplantation. †: death. ✢: killed. (c) The islets of diabetic NOD mice transplanted with NOD-IPCs were not stained by the anti-insulin antibody. NOD mice that did not develop diabetes were used as controls. H&E staining. Scale bar, 100 μm. (d) The number of insulin-positive islets was significantly lower in NOD-IPC-transplanted NOD mice than in naïve NOD mice. The lines in the boxes show the median values (*P < 0.05, Mann–Whitney U test). (e) Serum insulin concentration in NOD mice transplanted with NOD-IPCs. The lines in the boxes show the median values (*P < 0.05, one-way ANOVA followed by the Tukey–Kramer test). (f) Serum C-peptide concentration in NOD mice transplanted with NOD-IPCs. The lines in the boxes show the median values (*P < 0.05, one-way ANOVA followed by the Tukey–Kramer test).