Table 3 Primers and probes for parasite density qPCR.

From: Deletions of the Plasmodium falciparum histidine-rich protein 2/3 genes are common in field isolates from north-eastern Tanzania

Primers/Probes

5′ Modification

Sequence

3′ Modification

PgMET_F

 

5′-TGAAAGCAGCGTAGCTCAGA

 

PgMET_R

 

5′-CGCGTGGTTTCGATCCACG

 

PgMET_pr

FAM

5′-GGGGCTCATAACCCCCAGGA

BHQ2

HumTuBB_F

 

5′-AAGGAGGTCGATGAGCAGAT

 

HumTuBB_R

 

5′-GCTGTCTTGACATTGTTGGG

 

HumTuBB_pr

JOE

5′-TTAACGTGCAGAACAAGAACAGCAGCT

BHQ2