Figure 2 | Scientific Reports

Figure 2

From: Engineering and validation of a dual luciferase reporter system for quantitative and systematic assessment of regulatory sequences in Chinese hamster ovary cells

Figure 2The alternative text for this image may have been generated using AI.

Analysis of promoter strength using the dual luciferase reporter system in transient expression settings. The activities of nine promoters was evaluated by dual luciferase assay at 48 h post-transfection. Fluc expression is driven by the variable promoter and enables the systematic comparison of promoter activities, while the Rluc signal is an internal control regulated by the invariable CMV-mIE promoter. The measurement of promoter strength based on the ratio of Fluc to Rluc signals was investigated in (A) CHO-WT, (B) CHO-DG44 adherent, (C) CHO-DG44 suspension, and (D) HEK-293T cell lines. The CMV-mIE promoter containing vector exhibited the highest value which was normalized to 100 in all cell lines. (E) Cross comparison of promoter activities across cell lines was examined by setting the CMV-mIE promoter as a standard. The relative activity in HEK-293T cell line was set at 100.

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