Figure 7
D1-like receptor stimulation alters cytokine secretion of PBMCs from female RA patients compared to HC. PBMCs from HC (n = 13–12) and RA patients (n = 13–12) were stimulated with CpG and indicated concentrations of D1-like receptor agonists A68930 and SKF38393 for 24 h in vitro. Supernatants were stored at -80 °C for subsequent analysis of IL-8 and CCL3 concentrations by ELISA. (a, c) Absolute IL-8 (a) and CCL3 (c) concentrations in supernatants from unstimulated and CpG-stimulated PBMCs from HC and RA patients are shown. Lines indicate median with SD. (b, d) IL-8 (b) and CCL3 (d) concentrations in supernatants after D1-like stimulation were normalized to CpG controls from HC and RA patients and are presented as relative changes on a logarithmic scale. Mixed-effects-analysis with Sidak multiple comparison test was used to analyze cytokine secretion between unstimulated and CpG stimulated samples from HC and RA; Raw data of cytokine concentrations were logarithmized and analyzed by mixed-effects analysis with Geisser-Greenhouse correction and Dunnett multiple comparison test to determine the influence of D1-like receptor stimulation on cytokine secretion within HC and RA group; *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001.
