Figure 12

Immunostaining images of (a) Cytokeratin 18 and (b) ZO-1 of ARPE cells at day 7. These images indicated the monolayer sheet formation by ARPE cells but most of ZO-1 protein was observed in intracellular pools. (c) Expression of RPE65, and CRALBP genes in ARPE cells cultured for 7 days and from passage 5 (P5) and passage 20 (P20), detected by RT-PCR ((c) has been cropped and its original images are shown in the Supplementary Information file).