Figure 2
From: Organocatalyst treatment improves variant calling and mutant detection in archival clinical samples
Percentage of SNPs in DNA from 12-h or 72-h formalin-fixed paraffin-embedded (FFPE) tissue samples with or without organocatalyst (ORG∆) treatment relative to paired frozen (FR) DNA samples, classified according to type of change. (A) Percent of SNPs identified in FFPE (left) or ORG∆ FFPE (right) relative to matched FR DNA and classified as unique (present in FFPE and not FR), concordant (present in FR and FFPE), zygosity change (predicted to be a homozygous SNP in FFPE but heterozygous in FR or vice versa), and genotype change (predicted to be one genotype in FFPE but a different genotype in FR). (B) SNPs identified in FFPE (left) or ORG∆ FFPE (right) relative to matched FR DNA and sub-classified by zygosity change as heterozygous to homozygous (hetero_to_homo) or homozygous to heterozygous (homo_to_hetero). Zygosity changes are FFPE relative to FR. (C) Genotype changes between FFPE DNA SNPs compared to FR DNA SNPs. Genotype changes are classified according to their specific genotypes (INS insertion, DEL deletion).
