Figure 2

DNA methylation and mRNA expression of the Fxyd1 isoforms in the brain. (a) Fxyd1a gene promoter structure showing the analyzed CpGs. Seven CpG sites were analyzed at Fxyd1a promoter. The numbers of the CpGs are referred to the transcription starting site (TSS) indicated with the black arrow at + 1. Black arrows at the top specify the position of the primers used for bisulfite amplification. (b) Fxyd1b gene promoter structure showing the analyzed CpGs. Eight CpG sites were analyzed at Fxyd1b gene. The numbers of the CpGs refer to the transcription starting site (TSS) indicated with the black arrow at + 1. Black arrows at the top specify the position of the primers used for bisulfite amplification. (c) Fxyd1a average methylation (%) showed at P1, P15 and P60 developmental stages. Comparisons between developmental stages were performed using One-way ANOVA followed by Multiple t test. (d) Average methylation at single CpG sites displaying the methylation trend in the different developmental stages at Fxyd1a promoter. (e) mRNA expression levels of Fxyd1a referred to each developmental stage. Fxyd1a mRNA expression was normalized to the mean of the housekeeping gene and is expressed as 2^–ΔCt values. Comparisons between developmental stages were performed using One-way ANOVA followed by Multiple t test. (f) Pearson correlation between Fxyd1a mRNA expression and Fxyd1a promoter methylation at all analyzed developmental stages. (g) Fxyd1b average methylation (%) is showed at P1, P15 and P60 developmental stages. (h) Average methylation at each of analyzed CpG sites at Fxyd1b locus at the different developmental stages. (i) Expression levels of Fxyd1b referred to each developmental stage. Fxyd1b mRNA expression was normalized to the mean of the housekeeping gene and is expressed as 2^–ΔCt values. Comparisons between developmental stages were performed using One-way ANOVA followed by Multiple t test.*p < 0.05.