Figure 3
From: Tmem174, a regulator of phosphate transporter prevents hyperphosphatemia
Characteristics of Tmem174−/− mice. Expression of Tmem174 mRNA and protein in Tmem174+/+, Tmem174+/-, and Tmem174−/− mice. PCR and Western blotting analysis in the kidney of mice. (a) Aliquots of each PCR product were electrophoresed on a 1.5% agarose gel. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as the internal control. (b) Western blotting analysis of BBMVs isolated from the kidneys of Tmem174+/+, Tmem174+/-, and Tmem174−/− mice. Each lane was loaded with 20 μg of BBMV. Actin was used as the internal control. (c) Growth curves for male and female Tmem174+/+, Tmem174+/-, and Tmem174−/− mice. Values are mean ± SE (n = 10–30). *p < 0.05 vs. Tmem174+/+, #p < 0.05 vs. Tmem174+/- mice. Metabolic cages were used for measurement of 24-h food intake (g/day), and collection of urine, and feces from mice. (d) Food intake, (e) plasma creatinine, (f) plasma blood urea nitrogen, (g) blood ionized Ca, (h) fecal Ca excretion, (i) urinary Ca excretion, (j) plasma Pi, (k) fecal Pi excretion, (l) urinary Pi excretion. Male Tmem174+/+, Tmem174+/-, and Tmem174−/− mice at 8–9 weeks of age (n = 30–50). Values are mean ± SE.
