Figure 6
From: Tmem174, a regulator of phosphate transporter prevents hyperphosphatemia
Deletion of Tmem174 and renal NaPi transporters. (a) Western blot analysis of NaPi transporters in Tmem174+/+ and Tmem174−/− mice (8-week-old mice, n = 5, respectively). Each lane was loaded with 20 μg of BBMVs. Actin was used as an internal control. Values are mean ± SE. #’p < 0.01. Experiments were repeated at least 3 times. (b) Immunofluorescence staining of NaPi2a or NaPi2c (green) in renal sections of 8-week-old Tmem174+/+ and Tmem174−/− mice. DAPI (blue), Villin (red). Sections were prepared from kidneys embedded in the OCT compound and frozen. Scale bar; 100 μm. (c,d) Western blotting analysis of FGFR, α-Klotho (c), and total ERK1/2/phosphorylation ERK1/2 (d) levels. Each lane was loaded with 20 μg of cortical membranes (c) or whole homogenate (d). Actin was used as an internal control. Values are mean ± SE. #’p < 0.01. Experiments were repeated at least 3 times.
