Figure 8
From: Tmem174, a regulator of phosphate transporter prevents hyperphosphatemia
Dietary Pi regulation in Tmem174−/− mice. Male Tmem174+/+ and Tmem174−/− mice (n = 5) fed a low Pi (LP: 0.02%), control Pi (CP: 0.6%), or high Pi (HP: 1.2%) diet for 12 days. (a) Plasma Pi, (b) Serum intact FGF23, Values are mean ± SE. #p < 0.05, #’p < 0.01 vs same diet, ap < 0.05, a’p < 0.01 vs LP, b’p < 0.01 vs. CP of same genotype. (c) Western blotting analyses. Each lane was loaded with 20 μg of BBMVs. Actin was used as an internal control. Values are mean ± SE. #p < 0.05, #’p < 0.01. (d) Immunostaining of renal NaPi2a (Green) localization in the kidney sections of mice fed the LP (0.02% Pi) and HP (1.2% Pi) diet for 7 days. DAPI (Blue), Villin (Red). Experiments were performed in triplicate.
