Table 1 List of the primers used in this study.
Primer name | Seq (5ʹ–3́ʹ) | Tm (°C) | Reference | Product size | Region within SARS-CoV-2 gene | Regions in SARS-CoV-2 genome |
|---|---|---|---|---|---|---|
JUST_E1_F | ATTCGTTTCGGAAGAGACAGG | 63 | This study | 117 | 02–29 | 26,254–26,274 |
JUST_E1_R | CGCACACAATCGAAGCGC | 62.3 | This study | 106–123 | 26,350–26,367 | |
JUST_N1_F | ACCCAATAATACTGCGTCTTGG | 63.5 | This study | 138 | 135–156 | 28,409–28,430 |
JUST_N1_R | GGTAGCTCTTCGGTAGTAGCC | 64.4 | This study | 253–273 | 28,527–28,547 | |
JUST_RdRp1_F | GTACTGATGTCGTATACAGGGC | 63 | This study | 104 | 79–100 | 13,255–13,276 |
JUST_RdRp1_R | CTTCGTCCTTTTCTTGGAAGCG | 64.7 | This study | 162–183 | 13,338–13,359 | |
JUST_S1_F | ACAACCAGAACTCAATTACCCC | 63.5 | This study | 66 | 55–76 | 21,617–21,638 |
JUST_S1_R | TGTCAGGGTAATAAACACCACG | 63.3 | This study | 100–121 | 21,663–21,684 | |
GAPDH_F | CAATGACCCCTTCATTGACC | 61.7 | Tsubouchi et al. (2017)21 | 159 | ||
GAPDH_R | TTGATTTTGGAGGGATCTCG | 60.6 | Tsubouchi et al. (2017)21 | |||
β-actin F | CCCAAGGCCAACCGCGAGAAGAT | 61.4 | Law et al. (2005)22 | 219 | ||
β-actin R | GTCCCGGCCAGCCAGGTCCAG | 61.3 | Law et al. (2005)22 | |||
