Table 2 Results of samples for crude and column-based RNA extraction methods.
SYBR Green-based protocol | TaqMan based multiplex real-time PCR assay | No. of samples | Amplified for S gene |
|---|---|---|---|
Result of crude RNA extraction method | |||
Positive | Positive | 49 | – |
Negative | Negative | 8 | – |
Negative | Positive | 6 | 2 |
Positive | Negative | 27 | 9 (undetermined 8a) |
Total | 90 | ||
Result of column-based RNA extraction method | |||
Positive | Positive | 61 | – |
Negative | Negative | 25 | – |
Negative | Positive | 2 | 0 (undetermined 1a) |
Positive | Negative | 2 | 0 |
Total | 90 | ||
