Table 3 Operating characteristics of in-house SYBR green assay.

From: Development and validation of cost-effective one-step multiplex RT-PCR assay for detecting the SARS-CoV-2 infection using SYBR Green melting curve analysis

Extraction method

Sensitivity

Specificity

PPV

NPV

LR + 

LR-

Kappa index

Quick extract (n = 90)

89.09 (96.67a)

22.86 (54.55a)

64.47 (85.29a)

57.14 (85.71a)

1.15 (2.13a)

0.48(0.06a)

0.134 (0.579a)

Column based extraction (n = 90)

96.83 (100b)

92.59 (92.86b)

96.86 (96.83b)

92.59 (100b)

13.07 (14.0b)

0.03 (0.0b)

0.894 (0.947b)

  1. PPV positive predictive value, NPV negative predictive value, LR + positive likelihood ratio, LR − negative likelihood ratio.
  2. aConsidering Sansure for condodant samples and S gene amplification for contradictory false-positive and negative samples as the standard, respectively. The sample number here would be 82 (n = 82) due to undetermined results of eight samples in spike amplification step.
  3. bConsidering Sansure as standard for 86 concordant samples and S gene amplification as standard for the four contradictory samples. The sample number would be 89 (n-89) here due to one undetermined result in PCR and gel run.
Back to article page