Figure 5
From: Proteome-wide landscape of solubility limits in a bacterial cell
Protein stickiness and disorder content shape solubility. (A) We use the surface stickiness score to measure promiscuous interaction propensity38. Both fluorescent and dark aggregates show higher surface stickiness than soluble proteins (P < 10–10 and P < 10–10 respectively, Wilcoxon Rank Sum test). (B) Fractions of aggregating proteins as a function of disorder content (binned data), calculated using PONDR VSL2B. Upper panel shows the fraction of proteins in fluorescent aggregates among those that are either soluble or in fluorescent aggregates, while the lower panel shows the fraction of proteins in dark aggregates among those that are either soluble or in dark aggregates. (C) Disorder content as a function of native protein abundance in E. coli based on41. The most abundant 20% of E. coli proteins have a significantly higher disorder content than those in the least abundant 20% bin (P = 4.18*10–11, Wilcoxon rank sum test). Disorder content was calculated using PONDR VSL2B, but similar results are obtained with other predictors (see Tables S6 and S10).
