Figure 4

Functional validation. (A) Representative Western blot images and statistical results for expression levels of signal transduction and activator of transcription 1 (STAT1) and nuclear factor (NF)-κB in heat-killed tuberculosis (HKTB)-stimulated classically activated macrophages (Ms) and tumor-educated macrophages (TEMs) before and after HKTB stimulation. GAPDH was used as an internal control. Measurement of levels of (B) proinflammatory cytokines and (C) anti-inflammatory cytokines released by Ms and TEMs before and after HKTB stimulation. Statistical analyses were performed by Student's t-test. ns, not significant.