Table 1 Half-maximum effective concentration (EC50) values of test articles on changes in intracellular calcium levels in cells expressing MRGPRX2 or its mutant.

From: Determination of key residues in MRGPRX2 to enhance pseudo-allergic reactions induced by fluoroquinolones

Test article

EC50 (µg/mL) ± SD

(P value)

Human

Dog

Wild type

F78Y

M109W

F78Y/M109W

Wild type

Compound 48/80

5.4 ± 0.57

4.8 ± 2.1

(P = 0.60)

2.6 ± 1.0**

(P = 0.0028)

1.3 ± 0.69**

(P < 0.001)

2.1 ± 0.93**

(P < 0.001)

CPFX

47 ± 6.1

33 ± 4.7*

(P = 0.010)

20 ± 8.4**

(P = 0.0018)

6.1 ± 1.8**

(P < 0.001)

12 ± 1.9**

(P < 0.001)

GFLX

360 ± 160

250 ± 69

(P = 0.24)

100 ± 16*

(P = 0.018)

74 ± 27*

(P = 0.013)

97 ± 37*

(P = 0.019)

LVFX

650 ± 330

500 ± 350

(P = 0.57)

210 ± 36*

(P = 0.038)

180 ± 140*

(P = 0.038)

90 ± 47*

(P = 0.015)

  1. Statistical significance was determined by unpaired t-test. Data are represented as mean ± S.D. of quadruplicate assays.
  2. CPFX ciprofloxacin, GFLX gatifloxacin, LVFX levofloxacin.
  3. *P < 0.05, **P < 0.01 vs. wild type human MRGPRX2.
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