Figure 8

Knockdown of ADAM12 gene expression inhibited Notch and TGF-β signalling pathway activation in hepatocellular carcinoma cells. (A, B) Relative protein expression levels of Notch signalling pathway markers (Notch2, Jagged1, and Hes1) and a TGF-β signalling pathway marker (TGF-β) were determined by Western blotting. (C) The expression of ADAM12 was not affected in cells treated with different concentrations of DAPT inhibitors for 48 h (DAPT, 30 μM, 50 μM, or 80 μM). (D) Compared with the control group, the DAPT inhibitor group exhibited inhibited liver cancer cell proliferation (DAPT, 50 μM). Magnification, 100 × ; scale bar, 100 μm. The data are presented as the mean ± SD of three independent experiments. The display of cropped gels and blots in the picture and original blots/gels are presented in Supplementary PDF. *p < 0.05, **p < 0.01 and ***p < 0.001 indicate significance.