Figure 2
NAD(P)H fluorescence lifetime imaging of healthy explanted murine duodenum. (a) Representative NAD(P)H fluorescence sum image (500 × 500 × 100 µm3), fluorescence lifetime image, enzyme map and activity map of healthy duodenum. (b) Segmentation of intestinal villi in epithelium and lamina propria using the same algorithm as described in Fig. 1. (c) Masked enzyme and activity maps for epithelium and lamina propria. (d) Frequency graph of preferential enzymatic activity in epithelium (data from n = 6 mice). (e) Frequency graph of enzymatic activity in lamina propria (data from n = 7 mice). Scale bar = 250 µm. (f) Pixel frequencies of anaerobic glycolysis-like, oxidative phosphorylation (oxPhos)/aerobic glycolysis-like pathways and oxidative burst, all given in percentage, for epithelial cells and lamina propria encompassing all mice in (d,e). The superimposed false color bar for each column indicates the corresponding general metabolic activity per pixel, in percent. Statistical analysis in (f) was performed using ANOVA test (ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001). Figure generated with Paint.NET, https://www.getpaint.net/download.html.
