Figure 3
NAD(P)H fluorescence lifetime imaging of murine host duodenum from mice infected with H. polygyrus. (a) Representative NAD(P)H fluorescence sum images (500 × 500 × 100 µm3) of host tissue (villi) at day 6, 10 and 14 after infection (masks generated by segmentation). Corresponding enzyme maps (b) and metabolic maps (c) of the NAD(P)H fluorescence sum images in (a). Scale bar for all images = 250 µm. (d) General metabolic activity of infected mice as compared to healthy mice (n = 2 mice for day 6; n = 3 mice for day 10; n = 4 mice for day 14 and n = 7 healthy mice—Fig. 2). (e) Frequency graphs for the three time points during infection encompassing data from the same infected mice as in (d). (f) Pixel frequencies of anaerobic glycolysis-like pathways, oxidative phosphorylation (oxPhos)/aerobic glycolysis-like pathways and oxidative burst, all given in percentage, for villi tissue (including epithelium and lamina propria, without further segmentation) encompassing all mice in (e) and Fig. 2d,e. The superimposed false color bar for each column indicates the corresponding general metabolic activity per pixel, in percent. Statistical analysis in (d,f) was performed using ANOVA test with Bonferroni multi-column post-test (ns p > 0.05, *p < 0.05, **p < 0.01, ***p < 0.001). Figure generated with Paint.NET, https://www.getpaint.net/download.html.
