Figure 2
From: Direct observation of DNA alterations induced by a DNA disruptor
Conductance plots for FTD-incorporated DNA of transcription factor, p53, binding regions. (a) Heat maps of DNA conductance plots of DNA motif for p53 binding extracted from colorectal cancer line RKO (P). (b) Heat maps of synthesized DNA motif for p53 binding, wherein #6 and #16 thymines are non-fluorinated. (c) Heat maps of DNA conductance plots of synthesized DNA motif for p53 binding wherein #6 and #16 thymines are fluorinated. The x and y axes are the base position and conductance normalized to the conductance of guanine, respectively. (d) Enlarged conductance plots of the #16 position thymine for non-fluorinated (left), captured DNA motif for p53 binding for P (second left), captured DNA motif for p53 binding for F1 (middle), captured DNA motif for p53 binding for F5 (second right), and fluorinated DNA motif for p53 binding (right). (e, f) show each FTD incorporation rate in p53-binding motif DNA for RKO and HCT-116 cell lines, respectively. In the second column, the sequences neighboring of the FTD-incorporated position (#6, #16) are shown. In the third column, the conductance histograms relative to those of guanine are shown. The black and red lines represent the typical relative conductance values for T and FTD, respectively (Table 1). (g) FTD-exposure time dependency on FTD incorporation rates for RKO. (h) FTD-exposure time dependency on FTD conversion rates for HCT-116. FTD, trifluridine.
