Figure 3

FTD incorporation of DNA and anti-p53 immunoprecipitation. (a) Dependency of FTD incorporation rates on the consensus-binding region of TF, i.e., c-Myc (orange), NFKB3 (purple), and p53 (green). Based on RNA analysis, the p53 and NFKB3 were impaired by FTD exposure, while c-Myc was not impaired by FTD exposure. The FTD rates for p53 and NFKB3 are larger than those for c-Myc. TRRUST, transcriptional regulatory relationships detected by sentence-based text-mining; FTD, trifluridine; TF, transcription factor. (b) Flowchart of the preparation of “immunoprecipitated DNA” and “supernatant DNA” by anti-p53 immunoprecipitation. To confirm the inhibition of binding ability with p53 to the binding motif, we prepared immunoprecipitated DNA using anti-p53 antibody, which was extracted from RKO cell lines, and the DNA that does not bind to anti-p53 antibody, which was the supernatant of the immunoprecipitated using anti-p53 antibody. (c) FTD incorporation rate for the p53 binding domain in the immunoprecipitated DNA using anti-p53 antibody (right), supernatant after immunoprecipitation using anti-p53 antibody (left).