Table 2 Primers and references for specific protocols for amplification of the four DNA fragments sequenced in the study.
DNA fragment | Primer name | Primer direction | Primer sequence (5′-3′) | Primer source | PCR programme |
|---|---|---|---|---|---|
18S rRNA | 18S_Tar_Ff1 | Forward | AGGCGAAACCGCGAATGGCTC | ||
18S_Tar_Rr1 | Reverse | GCCGCAGGCTCCACTCCTGG | |||
28S rRNA | 28S_Eutar_F | Forward | ACCCGCTGAACTTAAGCATAT | ||
28SR0990 | Reverse | CCTTGGTCCGTGTTTCAAGAC | |||
ITS-2 | ITS2_Eutar_Ff | Forward | GCATCGATGAAGAACGCAGC | ||
ITS2_Eutar_Rr | Reverse | TCCTCCGCTTATTGATATGC | |||
COI | LCO1490 | Forward | GGTCAACAAATCATAAAGATATTGG | ||
HCOoutout | Reverse | GTAAATATATGRTGDGCTC |
