Figure 2
From: Temperature-sensitive migration dynamics in neutrophil-differentiated HL-60 cells
Temperature- and ECM-dependent direction correlation of differentiated HL-60 cell migration. (a–c) Direction autocorrelation analysis of differentiated HL-60 cells under 3 different conditions (control, 50% FBS and bFN coating) and 9 different temperatures between 30 and 42 °C. At hypothermic temperatures, directional autocorrelation peaks for all conditions. On bFN coating, cells maintain directionality for longer time periods. Error bars represent S.E.M. (d) Persistence length (µm) of differentiated HL-60 cells was calculated by multiplying the persistence time by average cell speed. The persistence time was evaluated by fitting Eq. (1) to the autocorrelation curves. At normal and hypothermic conditions, the persistence length was preserved. Error bars represent 95% CIs. (e) Average cell speed of differentiated HL-60 cells without coating (control), on 100 µg/ml bovine fibronectin coating (bFN coating), and on heat-treated bovine fibronectin coating (HT bFN coating). Heat treatment of the coating significantly reduced average cell speed. (f) Direction autocorrelation of differentiated HL-60 cells on control, bFN and HT bFN coatings at 37 °C. Heat treatment of bovine fibronectin did not influence cell persistence. Error bars are S.E.M. For all: 2 independent experiments were performed. **P < 0.01, ***P < 0.001, ****P < 0.0001.
