Figure 5
From: Temperature-sensitive migration dynamics in neutrophil-differentiated HL-60 cells
Differentiated HL-60 cell thermotaxis in response to temperature gradients in 3D microchambers. (a) Schematic representation of the 3D microfluidic temperature gradient chamber with cross sections in X and Y directions. Copper reservoirs were used as a heat source and a heat sink, with their temperatures held constant at 65 °C and 5 °C, respectively. The microchamber was filled with a collagen scaffold containing cells. (b) Migration tracks and polar histograms of θ for 20 cells each, migrating in 3D collagen matrix with and without temperature gradients. Application of the temperature gradients in 3D revealed a higher percentage of cells migrating towards the heat source. (c) Cell speed of differentiated HL-60 cells in a 3D collagen matrix at a control temperature of 37 °C and in the presence of the temperature gradient. The average speed of 20 cells in each condition was evaluated. For all: Error bars represent 95% CIs of the data. 2 independent experiments were performed. N.S. not significant.
