Figure 3
Induction of COX-2 by phosphorylated sphingoid bases and prostaglandins secretion. COX-2 protein levels increased after 24 h stimulation of human mesangial cells with IL-1β or PDGF-BB (a). PGE2 is secreted in the medium after both treatments (b), while PGI2 (measured through its metabolite 6-keto PGF1α) is secreted after the IL-1β treatment only (c). Thromboxane A2 (measured through its metabolite TXB2) secretion is not regulated (d). *P < 0.05; **P < 0.01. Data are reported as mean ± SEM. Uncropped blots after chemiluminescence development and total protein stain free blots are presented in Supplementary Fig. S4c. Quantification of the bands in the western blot is reported in Supplementary Fig. S5.
