Figure 4
Activation of rate-limiting enzyme cPLA2, upstream of COX-2. Phospholipase cPLA2 is upregulated and phosphorylated in human mesangial cells after 24 h stimulations with IL-1β and PDGF-BB (a). cPLA2 activity is accordingly increased (b). The activity in the controls was below the LOD of 3.5 nmol/min/ml. In this case, the LOD value was plotted in the graph. PLA2G4A gene expression was already upregulated after 4 h in both treatments (c, d). *P < 0.05; ***P < 0.001. Data are reported as mean ± SEM. Uncropped blots after chemiluminescence development and total protein stain free blots are presented in Supplementary Fig. S4d. Quantification of the bands in the western blot is reported in Supplementary Fig. S5.
