Figure 1
From: Biophysical and functional study of CRL5Ozz, a muscle specific ubiquitin ligase complex
Expression and purification of CRL5Ozz components. (a) Coomassie brilliant blue (CBB)–stained gels of baculovirus-produced Ozz (Plaque 1, Plaque 2), Elo B (Plaque 1, Plaque 2), Elo C (Plaque 1, Plaque 2), Cul 5 (Plaque 1, Plaque 2), and Rbx1 (Plaque 1, Plaque 2) from total lysates of individual plaques, compared to mock control (Control). (b) Western blot analysis of total lysates overexpressing the individual CRL5Ozz components probed with of anti-Ozz, anti-Elo B, anti-Elo C, anti-Cul5 and anti-Rbx1 specific antibodies. (c,d) CBB-stained SDS‐polyacrylamide gels of Ni-NTA agarose affinity purified Ozz–EloBC and Cul5–Rbx1 subcomplexes.
