Figure 4

Sedimentation velocity—AUC analysis of Ozz–EloBC, Cul5–Rbx1, and CRL5^Ozz complex. Panels (a–c) Top panel: Fringe displaced sedimentation velocity profiles (fringes vs radius), with superimposed solid lines showing the best fit to the model, and below residuals of the fits. Bottom panel: display of the continuous sedimentation coefficient distribution c(s) plots of Ozz–EloBC (red line), Cul5–Rbx1 (blue line), and a mixture of the former two complexes (black line); (d–f) Top panel: fringe displaced sedimentation velocity profiles (fringes vs radius), with superimposed solid lines showing the best fit to the model, and below residuals of the fits. Middle panel: Contour plots (heat maps) of the two-dimensional size-and-shape distributions, c(s,f/f₀) and Bottom panel: Molar mass distributions c(s,M), of Ozz–EloBC, Cul5–Rbx1 and a mixture of Ozz–EloBC and Cul5–Rbx1, respectively. The experiments were conducted in 137 mM NaCl, 0.27 mM KCl, 10 mM Na2HPO4 and 1.8 mM KH2PO4 pH 7.2 buffer at 20 °C and at a rotor speed of 50,000 rpm. The s-, f/f₀ and M-values of the protein complexes are listed in Tables 2 and 3.