Table 1 Face-centered central composite design matrix of four process variables for methyl red decolorization (%) and lipid production (%) by using Scenedesmus obliquus.

From: Statistical optimization for simultaneous removal of methyl red and production of fatty acid methyl esters using fresh alga Scenedesmus obliquus

Std	Run	Type	X₁	X₂	X₃	X₄	Methyl red decolorization (%)			Lipid production (%)
Std	Run	Type	X₁	X₂	X₃	X₄	Actual	Predicted	Residuals	Actual	Predicted	Residuals
1	1	Fact	− 1	− 1	− 1	− 1	29.63	29.31	0.32	18.47	18.44	0.03
7	2	Fact	− 1	1	1	− 1	57.27	56.67	0.60	18.34	18.25	0.09
25	3	Center	0	0	0	0	70.26	70.30	− 0.04	20.80	20.50	0.30
8	4	Fact	1	1	1	− 1	53.80	53.86	− 0.06	20.08	20.04	0.04
20	5	Axial	0	1	0	0	67.57	67.62	− 0.05	19.33	19.41	− 0.08
15	6	Fact	− 1	1	1	1	50.31	50.52	− 0.21	15.00	15.01	− 0.01
14	7	Fact	1	− 1	1	1	56.55	56.87	− 0.32	14.64	14.55	0.09
16	8	Fact	1	1	1	1	41.26	41.71	− 0.44	15.20	15.24	− 0.04
26	9	Center	0	0	0	0	70.50	70.30	0.20	20.57	20.50	0.07
24	10	Axial	0	0	0	1	67.24	66.10	1.14	17.16	17.26	− 0.10
28	11	Center	0	0	0	0	68.50	70.30	− 1.80	20.70	20.50	0.20
10	12	Fact	1	− 1	− 1	1	53.36	54.09	− 0.73	14.66	14.76	− 0.10
23	13	Axial	0	0	0	− 1	63.11	62.81	0.30	21.26	21.29	− 0.03
6	14	Fact	1	− 1	1	− 1	51.53	51.64	− 0.11	19.71	19.74	− 0.03
29	15	Center	0	0	0	0	68.40	70.30	− 1.90	20.34	20.50	− 0.16
27	16	Center	0	0	0	0	70.56	70.30	0.26	20.66	20.50	0.16
18	17	Axial	1	0	0	0	68.30	66.71	1.59	19.36	19.39	− 0.03
4	18	Fact	1	1	− 1	− 1	56.62	57.01	− 0.39	19.70	19.76	− 0.07
13	19	Fact	− 1	− 1	1	1	56.66	56.40	0.26	14.58	14.52	0.06
2	20	Fact	1	− 1	− 1	− 1	41.34	41.36	− 0.02	19.64	19.59	0.05
9	21	Fact	− 1	− 1	− 1	1	47.87	48.04	− 0.17	15.17	15.18	0.00
22	22	Axial	0	0	1	0	67.96	66.97	0.99	20.70	20.87	− 0.17
11	23	Fact	− 1	1	− 1	1	55.58	55.60	− 0.02	15.55	15.53	0.02
19	24	Axial	0	− 1	0	0	64.23	62.74	1.49	19.03	19.08	− 0.05
12	25	Fact	1	1	− 1	1	52.83	52.35	0.48	15.41	15.33	0.08
30	26	Center	0	0	0	0	69.27	70.30	− 1.03	20.33	20.50	− 0.17
17	27	Axial	− 1	0	0	0	64.94	65.09	− 0.15	18.60	18.70	− 0.10
21	28	Axial	0	0	− 1	0	64.81	64.36	0.45	21.10	21.06	0.04
5	29	Fact	− 1	− 1	1	− 1	44.45	45.16	− 0.72	18.12	18.16	− 0.04
3	30	Fact	− 1	1	− 1	− 1	54.33	54.25	0.08	18.36	18.41	− 0.05

	Variable	− 1	0	+ 1
X₁	Nitrogen source conc. (g/L)	0.5	1	1.5
X₂	Incubation time (days)	6	10	14
X₃	Initial pH level	7	8	9
X₄	Methyl red concentration (mg/L)	10	20	30

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Table 1 Face-centered central composite design matrix of four process variables for methyl red decolorization (%) and lipid production (%) by using Scenedesmus obliquus.

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