Table 4 Fit summary of the experimental results of CCD for methyl red decolorization (%) by using Scenedesmus obliquus.

From: Statistical optimization for simultaneous removal of methyl red and production of fatty acid methyl esters using fresh alga Scenedesmus obliquus

Sequential model sum of squares

Source

SS

Df

MS

F value

P value

Linear vs mean

198.25

4

49.56

0.41

0.7968

2FI vs linear

691.97

6

115.33

0.95

0.4820

Quadratic vs 2FI

2282.27

4

570.57

483.09

 < 0.0001*

Lack of fit tests

Source

SS

Df

MS

F value

P value

Linear

2987.05

20

149.35

152.20

 < 0.0001*

2FI

2295.08

14

163.93

167.06

 < 0.0001*

Quadratic

12.81

10

1.28

1.31

0.4053

Model summary statistics

Source

SD

R2

Adjusted R2

Predicted R2

PRESS

Linear

10.94

0.0621

− 0.0879

− 0.4248

4545.25

2FI

11.00

0.2790

− 0.1004

− 1.8497

9091.05

Quadratic

1.09

0.9944

0.9893

0.9819

57.76

  1. *Significant values, Df: degree of freedom, PRESS: sum of squares of prediction error, 2FI: two factors interaction, SD: Standard deviation, SS: Sum of Squares, MS: Mean Square.
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